Review



fluorescein isothiocyanate conjugated avidin fitc avidin  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Vector Laboratories fluorescein isothiocyanate conjugated avidin fitc avidin
    Fluorescein Isothiocyanate Conjugated Avidin Fitc Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pmc12736424-166-28-32
    Average 94 stars, based on 895 article reviews
    fluorescein isothiocyanate conjugated avidin fitc avidin - by Bioz Stars, 2026-09
    94/100 stars

    Images

    Related Articles

    Avidin-Biotin Assay:

    Article Title: Human centromere repositioning activates transcription and opens chromatin fibre structure
    Article Snippet: .. Detection of biotin label was performed with sequential layers of fluorescein (FITC)-conjugated avidin (Vector, A-2011; 1:500), biotinylated anti-avidin (Vector, BA-0300; 1:100) and a further layer of FITC-avidin (Vector, A-2011; 1:500). .. Digoxigenin was detected with sequential layers of Rhodamine-conjugated anti-digoxigenin (Roche, 11 207 750 910; 1:20) and Texas-Red (TR) –conjugated anti-sheep IgG (Vector, TI 6000; 1:100).

    Article Title: Human centromere repositioning activates transcription and opens chromatin fibre structure.
    Article Snippet: .. Detection of biotin label was performed with sequential layers of fluorescein (FITC)-conjugated avidin (Vector, A-2011; 1:500), biotinylated anti-avidin (Vector, BA-0300; 1:100) and a further layer of FITC-avidin (Vector, A-2011; 1:500). .. Digoxigenin was detected with sequential layers of Rhodamine-conjugated anti-digoxigenin (Roche, 11 207 750 910; 1:20) and Texas-Red (TR) –conjugated anti-sheep IgG (Vector, TI 6000; 1:100).

    Article Title: Enkephalinergic Circuit Involved in Nociceptive Modulation in the Spinal Dorsal Horn.
    Article Snippet: Enkephalin (ENK) has been implicated in pain modulation within the spinal dorsal horn (SDH).. Revealing the mechanisms underlying ENK analgesia entails the anatomical and functional knowledge of spinal ENK-ergic circuits.. Herein, we combined morphological and electrophysiological studies to unravel local ENK-ergic circuitry within the SDH.

    Article Title: Isolating and purifying cells for therapy
    Article Snippet: For staining of Lectin, cells were incubated with biotinylated UEA Lectin (Vector). .. Primary antibodies or lectin were then localized with FITC anti-rabbit, FITC anti-Mouse, or FITC avidin (Vector) at 1:100 dilution. ..

    Article Title: Isolating and purifying cells for therapy
    Article Snippet: Equivalent numbers of buffy coat derived leukocytes or column derived cells were incubated with the biotinylated anti-CD133 antibody 293 (Miltenyi, Auburn, Calif.) for 40 minutes. .. Cells were then rinsed in PBS and stained with FITC avidin (Vector Laboratories, Burlingame, Calif.) according to manufacturer's instructions. .. After being cytospun onto glass slides, cells were mounted with DAPIcontaining mouting media (Vector) and examined under fluorescent microscopy with dual color imaging.

    Article Title: Tac1-expressing neurons in the central amygdala predominantly mediate histamine-induced itch by receiving inputs from parabrachial Tac1-expressing neurons
    Article Snippet: .. This was followed by the addition of the secondary antibodies: alexa 594 donkey anti-rabbit (1:500, ab141637, Invitrogen), alexa 647 donkey anti-mouse (1:500, A31571, Invitrogen), and FITC Avidin (1:500, A − 2001, Vectorlabs). ..

    Incubation:

    Article Title: Identification and validation of Rab11a in Rat orofacial inflammatory pain model induced by CFA.
    Article Snippet: Orofacial pain (OFP) is a clinically very common and the most troubling condition; however, there is few effective way to relieve OFP.. Rab11a, a small molecule guanosine triphosphate enzyme, is one of the Rab member family playing a vital role in intracellular endocytosis and the pain process.. Therefore, we investigated the hub genes of rat OFP model induced by Complete Freund’s Adjuvant (CFA) via re-analyzing microarray data (GSE111160).

    Fluorescence In Situ Hybridization:

    Article Title: Central medial thalamic nucleus dynamically participates in acute itch sensation and chronic itch-induced anxiety-like behavior in male mice
    Article Snippet: Viruses and drugs used for anatomical or behavioral purposes 167 Experimental purposes Viruses/Drugs Location/ Target nuclei Volume Serot ype Serial Number Morphological FOS expression His/CQ/Saline nape 15 μl experiments Acetone/DNFB nape Retrograde FG right mPFC/CM 0.04 μl tracing 488-retrobeads Bilateral mPFC 150 nl Anterograde AAV-hSyn-eGFP-Synaptophysin-mRuby right PBN 150 nl AAV2/9 PT-0937 tracing AAV-cFos-eYFP right PBN 150 nl AAV2/9 PT-0129 AAV-hSyn-Cre-mCherry right PBN 150 nl AAV2/9 PT-0407 Behavioral Baseline His/CQ/Saline nape 15 μl experiments Acetone/DNFB nape Fiber photometry AAV-CaMKII-GCaMP6m CM 150 nl AAV2/9 PT-0111 AAV-hSyn-Cre right PBN 150 nl AAV2/1 PT-0241 AAV-hSyn-DIO-GCaMP7s CM 150 nl AAV2/9 PT-1421 Pharmacological AAV-CMVbGlobin-Cre-mCherry Right mPFC 150 nl AAV2/R T1108* manipulation AAV-hSyn-DIO-hM3Dq-eGFP CM 150 nl AAV2/9 PT-0152 AAV-hSyn-DIO-hM4Di-eGFP CM 150 nl AAV2/9 PT-0153 Optogenetic AAV-hSyn-DIO-GtACR-eGFP CM 150 nl AAV2/9 PT-1368 manipulation AAV-hSyn-DIO-eGFP CM 150 nl AAV2/9 PT-1103 AAV-mdlx-DIO-hM3Dq-eGFP Bilateral mPFC 150 nl AAV2/9 PT-2654 NR1 knockdown AAV-CMV-DIO-(EGFP-U6)-shRNA (NR1) Bilateral mPFC 150 nl AAV2/9 PT-5878 AAV-CMV-DIO-(EGFP-U6)-shRNA(scramble) Bilateral mPFC 150 nl AAV2/9 PT-2644 Electrophysiological AAV-mdlx-eGFP Bilateral mPFC 150 nl AAV2/9 PT-2651 experiments AAV-CaMKII-hChR2(H134R)-mCherry CM 150 nl AAV2/9 PT-0005 168 *: AAV-CMVbGlobin-Cre-mCherry were provided by Shanghai Taitool Bioscience Co. Ltd. Other 169 viruses were provided by Wuhan Brain VTA Co. Ltd. 170 171 172 173 174 175 176 177 178 179 180 181 Supplementary table 2. .. Antisera used for DAB reaction, FISH staining and immunofluorescent 182 staining 183 Groups Primary antisera Secondary antisera Tertiary antisera DAB reaction Mouse anti-FOS (1:500, ab11959, Abcam, MA, UK) Biotin-donkey anti-mouse (1:500, AP192B, Merck Millipore, CA, USA) ABCkit (1:200, PK-6101, Vectorlabs, CA, USA) GFAP/NeuN Rabbit anti-GFAP (1:500, ab7260, Abcam) Mouse anti-NeuN (1:500, mab377, Merck Millipore) Alexa 488 donkey anti-rabbit (1:500, A21206, Invitrogen, CA, USA) Alexa 594 donkey anti-mouse (1:500, A21203, Invitrogen) FOS/eYFP/DAPI Mouse anti-FOS (1:500, ab11959, Abcam) Alexa 594 donkey anti-mouse (1:500, A21203, Invitrogen) DAPI (1:500, sc-3598, Santacruz, TX, USA) VGluT2/FG/FOS VGluT2 riboprobes anti-digoxigenin sheep antibody (1:1500, 11-207-733-910, Roche Diagnostic, Basel, Switzerland) Rabbit anti-FG antibody (1:1000, AB153-I, Merck Millipore) mouse anti-FOS antibody (1:500, ab11959, Abcam) FITC-Avidin (1:500, A-2001, Vectorlabs) Alexa 647-donkey anti-rabbit (1:500, A31573, Invitrogen) Alexa 594-donkey anti-mouse (1:500, A21203, Invitrogen) eGFP/mRuby/FG Rabbit anti-FG antibody (1:1000, AB153-I, Merck Millipore) Alexa 647-donkey anti-rabbit (1:500, A31573, Invitrogen) eGFP/mRuby/CaMKII Mouse anti-CaMKII antibody (1:500, ab22609, Abcam) Alexa 647-donkey anti-mouse (1:500, A31571, Invitrogen) eGFP/mRuby/GAD67 Mouse anti-GAD67 antibody (1:500, MAB5406, Merck Millipore) Alexa 647-donkey anti-mouse (1:500, A31571, Invitrogen) Biocytin 647-avidin (1:500, S21374, Invitrogen) 184 185 186 187 188 189 190 191 192 193 194 195 Supplementary table 3. .. The company names and serial number of drugs used in experiments 196 Chemicals Company names Serial Number His Sigma, MO, USA H7250 CQ Sigma, MO, USA C6628 DAB Sigma, MO, USA D12384 1-fluoro-2,4-dinitrobenzene (DNFB) Sigma, MO, USA D1529 Clozapine N-oxide (CNO) Sigma, MO, USA C8032 488-retrobeads Lumafluor, New York, NY, USA Cas:78G180 tetrodotoxin (TTX) Enzo Life Sciences, Farmingdale, NY, USA BML-NA120 4-aminopyridine (4-AP) Sigma, MO, USA A78403 AP-5 Sigma, MO, USA A6553 Picrotoxin (PTX) Sigma, MO, USA R284556 CNQX Sigma, MO, USA C127 Biocytin Sigma, MO, USA B4261 Lanicemine Sigma, MO, USA SML0635 197 198 199 200

    Staining:

    Article Title: Central medial thalamic nucleus dynamically participates in acute itch sensation and chronic itch-induced anxiety-like behavior in male mice
    Article Snippet: Viruses and drugs used for anatomical or behavioral purposes 167 Experimental purposes Viruses/Drugs Location/ Target nuclei Volume Serot ype Serial Number Morphological FOS expression His/CQ/Saline nape 15 μl experiments Acetone/DNFB nape Retrograde FG right mPFC/CM 0.04 μl tracing 488-retrobeads Bilateral mPFC 150 nl Anterograde AAV-hSyn-eGFP-Synaptophysin-mRuby right PBN 150 nl AAV2/9 PT-0937 tracing AAV-cFos-eYFP right PBN 150 nl AAV2/9 PT-0129 AAV-hSyn-Cre-mCherry right PBN 150 nl AAV2/9 PT-0407 Behavioral Baseline His/CQ/Saline nape 15 μl experiments Acetone/DNFB nape Fiber photometry AAV-CaMKII-GCaMP6m CM 150 nl AAV2/9 PT-0111 AAV-hSyn-Cre right PBN 150 nl AAV2/1 PT-0241 AAV-hSyn-DIO-GCaMP7s CM 150 nl AAV2/9 PT-1421 Pharmacological AAV-CMVbGlobin-Cre-mCherry Right mPFC 150 nl AAV2/R T1108* manipulation AAV-hSyn-DIO-hM3Dq-eGFP CM 150 nl AAV2/9 PT-0152 AAV-hSyn-DIO-hM4Di-eGFP CM 150 nl AAV2/9 PT-0153 Optogenetic AAV-hSyn-DIO-GtACR-eGFP CM 150 nl AAV2/9 PT-1368 manipulation AAV-hSyn-DIO-eGFP CM 150 nl AAV2/9 PT-1103 AAV-mdlx-DIO-hM3Dq-eGFP Bilateral mPFC 150 nl AAV2/9 PT-2654 NR1 knockdown AAV-CMV-DIO-(EGFP-U6)-shRNA (NR1) Bilateral mPFC 150 nl AAV2/9 PT-5878 AAV-CMV-DIO-(EGFP-U6)-shRNA(scramble) Bilateral mPFC 150 nl AAV2/9 PT-2644 Electrophysiological AAV-mdlx-eGFP Bilateral mPFC 150 nl AAV2/9 PT-2651 experiments AAV-CaMKII-hChR2(H134R)-mCherry CM 150 nl AAV2/9 PT-0005 168 *: AAV-CMVbGlobin-Cre-mCherry were provided by Shanghai Taitool Bioscience Co. Ltd. Other 169 viruses were provided by Wuhan Brain VTA Co. Ltd. 170 171 172 173 174 175 176 177 178 179 180 181 Supplementary table 2. .. Antisera used for DAB reaction, FISH staining and immunofluorescent 182 staining 183 Groups Primary antisera Secondary antisera Tertiary antisera DAB reaction Mouse anti-FOS (1:500, ab11959, Abcam, MA, UK) Biotin-donkey anti-mouse (1:500, AP192B, Merck Millipore, CA, USA) ABCkit (1:200, PK-6101, Vectorlabs, CA, USA) GFAP/NeuN Rabbit anti-GFAP (1:500, ab7260, Abcam) Mouse anti-NeuN (1:500, mab377, Merck Millipore) Alexa 488 donkey anti-rabbit (1:500, A21206, Invitrogen, CA, USA) Alexa 594 donkey anti-mouse (1:500, A21203, Invitrogen) FOS/eYFP/DAPI Mouse anti-FOS (1:500, ab11959, Abcam) Alexa 594 donkey anti-mouse (1:500, A21203, Invitrogen) DAPI (1:500, sc-3598, Santacruz, TX, USA) VGluT2/FG/FOS VGluT2 riboprobes anti-digoxigenin sheep antibody (1:1500, 11-207-733-910, Roche Diagnostic, Basel, Switzerland) Rabbit anti-FG antibody (1:1000, AB153-I, Merck Millipore) mouse anti-FOS antibody (1:500, ab11959, Abcam) FITC-Avidin (1:500, A-2001, Vectorlabs) Alexa 647-donkey anti-rabbit (1:500, A31573, Invitrogen) Alexa 594-donkey anti-mouse (1:500, A21203, Invitrogen) eGFP/mRuby/FG Rabbit anti-FG antibody (1:1000, AB153-I, Merck Millipore) Alexa 647-donkey anti-rabbit (1:500, A31573, Invitrogen) eGFP/mRuby/CaMKII Mouse anti-CaMKII antibody (1:500, ab22609, Abcam) Alexa 647-donkey anti-mouse (1:500, A31571, Invitrogen) eGFP/mRuby/GAD67 Mouse anti-GAD67 antibody (1:500, MAB5406, Merck Millipore) Alexa 647-donkey anti-mouse (1:500, A31571, Invitrogen) Biocytin 647-avidin (1:500, S21374, Invitrogen) 184 185 186 187 188 189 190 191 192 193 194 195 Supplementary table 3. .. The company names and serial number of drugs used in experiments 196 Chemicals Company names Serial Number His Sigma, MO, USA H7250 CQ Sigma, MO, USA C6628 DAB Sigma, MO, USA D12384 1-fluoro-2,4-dinitrobenzene (DNFB) Sigma, MO, USA D1529 Clozapine N-oxide (CNO) Sigma, MO, USA C8032 488-retrobeads Lumafluor, New York, NY, USA Cas:78G180 tetrodotoxin (TTX) Enzo Life Sciences, Farmingdale, NY, USA BML-NA120 4-aminopyridine (4-AP) Sigma, MO, USA A78403 AP-5 Sigma, MO, USA A6553 Picrotoxin (PTX) Sigma, MO, USA R284556 CNQX Sigma, MO, USA C127 Biocytin Sigma, MO, USA B4261 Lanicemine Sigma, MO, USA SML0635 197 198 199 200

    Article Title: Isolating and purifying cells for therapy
    Article Snippet: Equivalent numbers of buffy coat derived leukocytes or column derived cells were incubated with the biotinylated anti-CD133 antibody 293 (Miltenyi, Auburn, Calif.) for 40 minutes. .. Cells were then rinsed in PBS and stained with FITC avidin (Vector Laboratories, Burlingame, Calif.) according to manufacturer's instructions. .. After being cytospun onto glass slides, cells were mounted with DAPIcontaining mouting media (Vector) and examined under fluorescent microscopy with dual color imaging.



    Similar Products

    fluorescein isothiocyanate conjugated avidin fitc avidin  (Vector Laboratories)
    94
    Vector Laboratories fluorescein isothiocyanate conjugated avidin fitc avidin
    Fluorescein Isothiocyanate Conjugated Avidin Fitc Avidin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pmc12736424-166-28-32
    Average 94 stars, based on 1 article reviews
    fluorescein isothiocyanate conjugated avidin fitc avidin - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    fitc avidin  (Thermo Fisher)
    94
    Thermo Fisher fitc avidin
    Fitc Avidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Paraformaldehyde%2C+90%25%2C+pure/pmc13181568-56-4-11
    Average 94 stars, based on 1 article reviews
    fitc avidin - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    avidin fitc  (Thermo Fisher)
    96
    Thermo Fisher avidin fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Avidin/pmc12834844-154-22-23
    Average 96 stars, based on 1 article reviews
    avidin fitc - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    fitc conjugated avidin  (Thermo Fisher)
    99
    Thermo Fisher fitc conjugated avidin
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Fitc Conjugated Avidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Avidin/pm41378780-79-5-11
    Average 99 stars, based on 1 article reviews
    fitc conjugated avidin - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    avidin fitc  (Vector Laboratories)
    94
    Vector Laboratories avidin fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pm41375453-68-6-7
    Average 94 stars, based on 1 article reviews
    avidin fitc - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    avidin conjugated fluorescein isothiocyanate fitc  (Vector Laboratories)
    94
    Vector Laboratories avidin conjugated fluorescein isothiocyanate fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Conjugated Fluorescein Isothiocyanate Fitc, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Fluorescein+Avidin+D/pmc12738967-378-38-42
    Average 94 stars, based on 1 article reviews
    avidin conjugated fluorescein isothiocyanate fitc - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier
    avidin conjugated to fluorescein fitc  (Thermo Fisher)
    99
    Thermo Fisher avidin conjugated to fluorescein fitc
    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show <t>avidin–FITC-positive</t> degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.
    Avidin Conjugated To Fluorescein Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fitc+avidin/Avidin/us12391729-2667-0-5
    Average 99 stars, based on 1 article reviews
    avidin conjugated to fluorescein fitc - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show avidin–FITC-positive degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.

    Journal: Biotechnology Reports

    Article Title: Production and characterization of rNGFSP: a recombinant fusion immunogen eliciting dual anti-NGF and anti-Substance P therapeutic antibodies for Degenerative Joint Disease

    doi: 10.1016/j.btre.2026.e00946

    Figure Lengend Snippet: Functional neutralization of NGF binding to the TrkA receptor and SP-induced mast cell degranulation by antibodies elicited by rNGFSP in dogs. A) Inhibition of NGF–TrkA binding. Neutralization of NGF binding to the TrkA receptor was assessed by ELISA. Biotinylated NGF was incubated with TrkA in the presence of pooled sera from non-immunized dogs at day 0 (D0), sera from rNGFSP-immunized dogs at day 56 (D56), or a commercial monoclonal anti-NGF antibody (0.5 µg/mL). NGF alone served as a positive control. Sera were diluted 1:10 prior to the assay.. * p < 0.05. B) Inhibition of SP-induced mast cell degranulation. Flow cytometry analysis of mast cells stimulated with SP conjugated to BSA in the presence of IgG from non-immunized (D0) or immunized (D56) dogs. Representative plots show avidin–FITC-positive degranulating cells. The percentage of degranulating mast cells from replicate experiments is summarized on the right. All data are expressed as mean ± SEM, with * p < 0.05 compared to Day 0, determined by two-way ANOVA followed by Tukey’s multiple comparisons test. C) Confocal microscopy of mast cell degranulation. Mast cells were stained with avidin–FITC (green) and DAPI (blue). SP or the positive control compound 48/80 (10 µM) induced granule release, whereas IgG from immunized dogs (D56) reduced SP-induced degranulation. Scale bar: 10 µm.

    Article Snippet: After stimulation, cells were centrifuged (1000 × g, 10 min, 4 °C), resuspended in 150 μL PBS, and stained with 2 μg/mL Avidin-FITC (Thermo Scientific, #A821) for 20 min at room temperature.

    Techniques: Functional Assay, Neutralization, Binding Assay, Inhibition, Enzyme-linked Immunosorbent Assay, Incubation, Positive Control, Flow Cytometry, Avidin-Biotin Assay, Confocal Microscopy, Staining